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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated C1QA Monoclonal antibody

C1QA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B10E3

Cat no : CL594-67063

Synonyms

C1QA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated C1QA Monoclonal antibody (CL594-67063)

    IF Staining of HepG2 using CL594-67063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 C1QA antibody (CL594-67063, Clone: 1B10E3 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67063 targets C1QA in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C1QA fusion protein Ag28622 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent, A chain
Calculated molecular weight 245 aa, 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC030153
Gene symbol C1QA
Gene ID (NCBI) 712
RRIDAB_2920081
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Protocols

Product Specific Protocols
IF protocol for CL594 C1QA antibody CL594-67063Download protocol
Standard Protocols
Click here to view our Standard Protocols