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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1QL1 Polyclonal antibody (15757-1-AP)

    WB analysis using 15757-1-AP (same clone as 15757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15757-1-AP (C1QL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1QL1 Polyclonal antibody (15757-1-AP)

    IHC staining of mouse cerebellum using 15757-1-AP (same clone as 15757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1QL1 Polyclonal antibody (15757-1-AP)

    IHC staining of mouse cerebellum using 15757-1-AP (same clone as 15757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using C1QL1 Polyclonal antibody (15757-1-AP)

    IF Staining of mouse cerebellum using 15757-1-AP (same clone as 15757-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using C1QL1 antibody (15757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15757-1-PBS in a different storage buffer formulation.

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C1QL1 Polyclonal antibody, PBS Only

C1QL1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 15757-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15757-1-PBS targets C1QL1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1QL1 fusion protein Ag8348 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent-like 1
Calculated molecular weight 258 aa, 26 kDa
Observed molecular weight30 kDa
GenBank accession numberBC008798
Gene Symbol C1QL1
Gene ID (NCBI) 10882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75973
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

C1QL1 (complement component 1, q subcomponent-like 1) is one of the C1q/tumor-necrosis-factor-related protein (CTRP) family members. C1QL1 is synthesized and secreted mainly by the brain and adipose tissues. It is implicated in synapse formation or elimination in cultured hippocampal neurons in vitro.