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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1RL Polyclonal antibody, PBS Only

C1RL Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24820-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1RL Polyclonal antibody (24820-1-AP)

    WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C1RL Polyclonal antibody (24820-1-AP)

    IF Staining of U2OS using 24820-1-AP (same clone as 24820-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24820-1-PBS targets C1RL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1RL fusion protein Ag20324 相同性解析による交差性が予測される生物種
Full Name complement component 1, r subcomponent-like
Calculated molecular weight 487 aa, 53 kDa
Observed molecular weight70 kDa
GenBank accession numberBC156780
Gene symbol C1RL
Gene ID (NCBI) 51279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).