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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C1qC Monoclonal antibody (66268-1-Ig)

    WB analysis of human plasma using 66268-1-Ig (same clone as 66268-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66268-1-Ig (C1QC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using C1qC Monoclonal antibody (66268-1-Ig)

    IF Staining of HUVEC using 66268-1-Ig (same clone as 66268-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using C1qC antibody (66268-1-Ig, Clone: 1C5B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

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C1qC Monoclonal antibody, PBS Only

C1qC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66268-1-PBS
Clone No.1C5B4

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C1Q C, C1qC, C1QG

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66268-1-PBS targets C1qC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen C1qC fusion protein Ag10233 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent, C chain
Calculated molecular weight 245 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC009016
Gene Symbol C1QC
Gene ID (NCBI) 714
RRIDAB_2881653
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP02747
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.