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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1qC Monoclonal antibody, PBS Only

C1qC Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5B4

Cat no : 66268-1-PBS

Synonyms

C1Q C, C1qC, C1QG

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C1qC Monoclonal antibody (66268-1-Ig)

    WB analysis of human plasma using 66268-1-Ig (same clone as 66268-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66268-1-Ig (C1QC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using C1qC Monoclonal antibody (66268-1-Ig)

    IF Staining of HUVEC using 66268-1-Ig (same clone as 66268-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using C1qC antibody (66268-1-Ig, Clone: 1C5B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66268-1-PBS targets C1qC in WB, IF, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen C1qC fusion protein Ag10233 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent, C chain
Calculated molecular weight 245 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC009016
Gene symbol C1QC
Gene ID (NCBI) 714
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.