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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    WB analysis of human plasma using 66242-1-Ig (same clone as 66242-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    IF Staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using C4 Gamma Chain antibody (66242-1-Ig, Clone: 2G6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

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C4 Gamma Chain Monoclonal antibody, PBS Only

C4 Gamma Chain Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66242-1-PBS
Clone No.2G6A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

C4A, C4 (gamma chain), C4A2, C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66242-1-PBS targets C4 Gamma Chain in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C4 Gamma Chain fusion protein Ag10449 相同性解析による交差性が予測される生物種
Full Name complement component 4A (Rodgers blood group)
Calculated molecular weight 1744 aa, 193 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC012372
Gene Symbol C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2881631
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0C0L4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4-A protein recognizes C4 gamma chain.