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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C4 Gamma Chain Monoclonal antibody

C4 Gamma Chain Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2G6A7

Cat no : 66242-1-Ig

Synonyms

Acidic complement C4, C4, C4 Gamma Chain, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    WB analysis of human plasma using 66242-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    IHC staining of human liver using 66242-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    IHC staining of human liver using 66242-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using C4 Gamma Chain Monoclonal antibody (66242-1-Ig)

    IF Staining of human liver using 66242-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using C4 Gamma Chain antibody (66242-1-Ig, Clone: 2G6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66242-1-Ig targets C4 Gamma Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C4 Gamma Chain fusion protein Ag10449 相同性解析による交差性が予測される生物種
Full Name complement component 4A (Rodgers blood group)
Calculated molecular weight 1744 aa, 193 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC012372
Gene symbol C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2881631
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4-A protein recognizes C4 gamma chain.

Protocols

Product Specific Protocols
WB protocol for C4 Gamma Chain antibody 66242-1-IgDownload protocol
IHC protocol for C4 Gamma Chain antibody 66242-1-IgDownload protocol
IF protocol for C4 Gamma Chain antibody 66242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols