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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C5 Polyclonal antibody (22492-1-AP)

    WB analysis using 22492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using C5 Polyclonal antibody (22492-1-AP)

    WB analysis using 22492-1-AP

    rat plasma was subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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C5 Polyclonal antibody

C5 Polyclonal Antibody for WB, ELISA
Cat No. 22492-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C5, C5b, C5, C5a, Complement C5 alpha chain, Complement C5, C5a anaphylatoxin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma, rat plasma, mouse serum

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22492-1-AP targets C5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5 fusion protein Ag17882 相同性解析による交差性が予測される生物種
Full Name complement component 5
Calculated molecular weight 1676 aa, 188 kDa
Observed molecular weight120 kDa, 70 kDa
GenBank accession numberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
RRIDAB_3669399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP01031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)

Protocols

Product Specific Protocols
WB protocol for C5 antibody 22492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols