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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C5 Polyclonal antibody (22492-1-AP)

    WB analysis using 22492-1-AP (same clone as 22492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using C5 Polyclonal antibody (22492-1-AP)

    WB analysis using 22492-1-AP (same clone as 22492-1-PBS)

    rat plasma was subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22492-1-PBS in a different storage buffer formulation.

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C5 Polyclonal antibody, PBS Only

C5 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 22492-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

C5, C5b, C5, C5a, Complement C5 alpha chain, Complement C5, C5a anaphylatoxin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22492-1-PBS targets C5 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5 fusion protein Ag17882 相同性解析による交差性が予測される生物種
Full Name complement component 5
Calculated molecular weight 1676 aa, 188 kDa
Observed molecular weight120 kDa, 70 kDa
GenBank accession numberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP01031
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)