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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated C5 Monoclonal antibody

C5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G8C9

Cat no : CL594-66634

Synonyms

C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated C5 Monoclonal antibody (CL594-66634)

    IF Staining of HepG2 using CL594-66634

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 C5 antibody (CL594-66634, Clone: 2G8C9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated C5 Monoclonal antibody (CL594-66634)

    FC experiment of HepG2 using CL594-66634

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human C5 (CL594-66634, Clone: 2G8C9) (red), or 0.4 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66634 targets C5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C5 fusion protein Ag17882 相同性解析による交差性が予測される生物種
Full Name complement component 5
Calculated molecular weight 1676 aa, 188 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC113740
Gene symbol C5
Gene ID (NCBI) 727
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 C5 antibody CL594-66634Download protocol
Standard Protocols
Click here to view our Standard Protocols