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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody, PBS Only

C5AR1/CD88 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241712D4

Cat no : 98226-1-PBS

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR)

    FC experiment of human peripheral blood leukocytes using 98226-1-RR (same clone as 98226-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98226-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR)

    FC experiment of human peripheral blood leukocytes using 98226-1-RR (same clone as 98226-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98226-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98226-1-PBS targets C5AR1/CD88 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name complement component 5a receptor 1
Calculated molecular weight39kDa
GenBank accession numberNM_001736.4
Gene symbol C5aR
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production.