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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody

C5AR1/CD88 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241712D4

Cat no : 98226-1-RR

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR)

    FC experiment of human peripheral blood leukocytes using 98226-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR)

    FC experiment of human peripheral blood leukocytes using 98226-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98226-1-RR targets C5AR1/CD88 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name complement component 5a receptor 1
Calculated molecular weight39kDa
GenBank accession numberNM_001736.4
Gene symbol C5aR
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production.

Protocols

Product Specific Protocols
FC protocol for C5AR1/CD88 antibody 98226-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols