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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C6orf15 Polyclonal antibody, PBS Only

C6orf15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24953-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf15 Polyclonal antibody (24953-1-AP)

    WB analysis using 24953-1-AP (same clone as 24953-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 24953-1-AP (C6orf15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C6orf15 Polyclonal antibody (24953-1-AP)

    IHC staining of mouse skin using 24953-1-AP (same clone as 24953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C6orf15 Polyclonal antibody (24953-1-AP)

    IHC staining of mouse skin using 24953-1-AP (same clone as 24953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using C6orf15 Polyclonal antibody (24953-1-AP)

    IF Staining of A375 using 24953-1-AP (same clone as 24953-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using C6orf15 antibody (24953-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24953-1-PBS targets C6orf15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C6orf15 fusion protein Ag20276 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 15
Calculated molecular weight 325 aa, 34 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC153157
Gene symbol C6orf15
Gene ID (NCBI) 29113
RRIDAB_2879818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.