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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C7 Monoclonal antibody, PBS Only

C7 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9C4

Cat no : 66908-1-PBS

Synonyms

Complement component C7, complement component 7, 1H9C4

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C7 Monoclonal antibody (66908-1-Ig)

    WB analysis of human plasma using 66908-1-Ig (same clone as 66908-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66908-1-Ig (C7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66908-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C7 Monoclonal antibody (66908-1-Ig)

    IF Staining of HepG2 using 66908-1-Ig (same clone as 66908-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C7 antibody (66908-1-Ig, Clone: 1H9C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66908-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using C7 Monoclonal antibody (66908-1-Ig)

    FC experiment of HepG2 using 66908-1-Ig (same clone as 66908-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug C7 Monoclonal antibody (66908-1-Ig, Clone:1H9C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66908-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66908-1-PBS targets C7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C7 fusion protein Ag12552 相同性解析による交差性が予測される生物種
Full Name complement component 7
Calculated molecular weight 843 aa, 94 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC063851
Gene symbol C7
Gene ID (NCBI) 730
RRIDAB_2882235
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.