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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C7 Monoclonal antibody

C7 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H9C4

Cat no : 66908-1-Ig

Synonyms

Complement component C7, complement component 7, 1H9C4

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C7 Monoclonal antibody (66908-1-Ig)

    WB analysis of human plasma using 66908-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66908-1-Ig (C7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C7 Monoclonal antibody (66908-1-Ig)

    IF Staining of HepG2 using 66908-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C7 antibody (66908-1-Ig, Clone: 1H9C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using C7 Monoclonal antibody (66908-1-Ig)

    FC experiment of HepG2 using 66908-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug C7 Monoclonal antibody (66908-1-Ig, Clone:1H9C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman plasma tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66908-1-Ig targets C7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C7 fusion protein Ag12552 相同性解析による交差性が予測される生物種
Full Name complement component 7
Calculated molecular weight 843 aa, 94 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC063851
Gene symbol C7
Gene ID (NCBI) 730
RRIDAB_2882235
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.

Protocols

Product Specific Protocols
WB protocol for C7 antibody 66908-1-IgDownload protocol
IF protocol for C7 antibody 66908-1-IgDownload protocol
FC protocol for C7 antibody 66908-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols