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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated C7 Monoclonal antibody

C7 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H9C4

Cat no : CL488-66908

Synonyms

Complement component C7, complement component 7, 1H9C4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated C7 Monoclonal antibo (CL488-66908)

    IF Staining of HepG2 using CL488-66908

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 C7 antibody (CL488-66908, Clone: 1H9C4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated C7 Monoclonal antibo (CL488-66908)

    FC experiment of HepG2 using CL488-66908

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated C7 Monoclonal antibody (CL488-66908, Clone:1H9C4)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66908 targets C7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C7 fusion protein Ag12552 相同性解析による交差性が予測される生物種
Full Name complement component 7
Calculated molecular weight 843 aa, 94 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC063851
Gene symbol C7
Gene ID (NCBI) 730
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 C7 antibody CL488-66908Download protocol
FC protocol for CL Plus 488 C7 antibody CL488-66908Download protocol
Standard Protocols
Click here to view our Standard Protocols