CoraLite®594-conjugated C9orf72 Monoclonal antibody
C9orf72 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3D2H6
Cat no : CL594-66140
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL594-66140 is C9orf72 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | C9orf72 fusion protein Ag21080 相同性解析による交差性が予測される生物種 |
Full Name | chromosome 9 open reading frame 72 |
Calculated molecular weight | 481 aa, 54 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC020851 |
Gene symbol | C9orf72 |
Gene ID (NCBI) | 203228 |
RRID | AB_2883523 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 C9orf72 antibody CL594-66140 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |