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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CA11 Recombinant antibody (83082-4-RR)

    IF Staining of U2OS using 83082-4-RR (same clone as 83082-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CA11 antibody (83082-4-RR, Clone: 230390F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83082-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using CA11 Recombinant antibody (83082-4-RR)

    FC experiment of A431 using 83082-4-RR (same clone as 83082-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CA11 Recombinant antibody (83082-4-RR, Clone:230390F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83082-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83082-4-RR

    Affinity and Kinetic Characterization of 83082-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-4-RR against Human CA11 were performed. The affinity constant is 0.78 nM.

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CA11 Recombinant antibody, PBS Only

CA11 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83082-4-PBS
Clone No.230390F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83082-4-PBS targets CA11 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CA11 fusion protein Ag33373 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XI
Calculated molecular weight 36 kDa
GenBank accession numberBC002662
Gene Symbol CA11
Gene ID (NCBI) 770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75493
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).