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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CA11 Recombinant antibody

CA11 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230390F7

Cat no : 83082-4-RR

Synonyms

Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CA11 Recombinant antibody (83082-4-RR)

    IF Staining of U2OS using 83082-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CA11 antibody (83082-4-RR, Clone: 230390F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using CA11 Recombinant antibody (83082-4-RR)

    FC experiment of A431 using 83082-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CA11 Recombinant antibody (83082-4-RR, Clone:230390F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83082-4-RR

    Affinity and Kinetic Characterization of 83082-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-4-RR against Human CA11 were performed. The affinity constant is 0.78 nM.

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83082-4-RR targets CA11 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CA11 fusion protein Ag33373 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XI
Calculated molecular weight 36 kDa
GenBank accession numberBC002662
Gene symbol CA11
Gene ID (NCBI) 770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

Protocols

Product Specific Protocols
IF protocol for CA11 antibody 83082-4-RRDownload protocol
FC protocol for CA11 antibody 83082-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols