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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CA11 Recombinant antibody

CA11 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230390F7

Cat no : CL488-83082-4

Synonyms

Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated CA11 Recombinant ant (CL488-83082-4)

    IF Staining of U2OS using CL488-83082-4

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 CA11 antibody (CL488-83082-4, Clone: 230390F7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated CA11 Recombinant ant (CL488-83082-4)

    FC experiment of A431 using CL488-83082-4

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CA11 Recombinant antibody (CL488-83082-4, Clone:230390F7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83082-4 targets CA11 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CA11 fusion protein Ag33373 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XI
Calculated molecular weight 36 kDa
GenBank accession numberBC002662
Gene symbol CA11
Gene ID (NCBI) 770
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CA11 antibody CL488-83082-4Download protocol
FC protocol for CL Plus 488 CA11 antibody CL488-83082-4Download protocol
Standard Protocols
Click here to view our Standard Protocols