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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CA12 Monoclonal antibody, PBS Only

CA12 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E1D8

Cat no : 68224-1-PBS

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA12 Monoclonal antibody (68224-1-Ig)

    WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CA12 Monoclonal antibody (68224-1-Ig)

    WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CA12 Monoclonal antibody (68224-1-Ig)

    IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68224-1-PBS targets CA12 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CA12 fusion protein Ag7235 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XII
Calculated molecular weight 39 kDa
Observed molecular weight38-45 kDa
GenBank accession numberBC000278
Gene symbol CA12
Gene ID (NCBI) 771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.