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CA12 Monoclonal antibody

CA12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68224-1-Ig
Clone No.3E1D8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68224-1-Ig targets CA12 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CA12 fusion protein Ag7235 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XII
Calculated molecular weight 39 kDa
Observed molecular weight38-45 kDa
GenBank accession numberBC000278
Gene Symbol CA12
Gene ID (NCBI) 771
RRIDAB_2935312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43570
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Protocols

Product Specific Protocols
WB protocol for CA12 antibody 68224-1-IgDownload protocol
IF protocol for CA12 antibody 68224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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