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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CA12 Monoclonal antibody

CA12 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E1D8

Cat no : CL488-68224

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated CA12 Monoclonal anti (CL488-68224)

    IF Staining of A549 using CL488-68224

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 CA12 antibody (CL488-68224, Clone: 3E1D8 ) at dilution of 1:1000.

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68224 targets CA12 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CA12 fusion protein Ag7235 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XII
Calculated molecular weight 39 kDa
Observed molecular weight38-45 kDa
GenBank accession numberBC000278
Gene symbol CA12
Gene ID (NCBI) 771
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CA12 antibody CL488-68224Download protocol
Standard Protocols
Click here to view our Standard Protocols