Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68224 targets CA12 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CA12 fusion protein Ag7235 相同性解析による交差性が予測される生物種 |
| Full Name | carbonic anhydrase XII |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 38-45 kDa |
| GenBank accession number | BC000278 |
| Gene Symbol | CA12 |
| Gene ID (NCBI) | 771 |
| RRID | AB_3672979 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43570 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CA12 antibody CL488-68224 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |