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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CACYBP Monoclonal antibody

CACYBP Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C2G11

Cat no : 68161-1-Ig

Synonyms

CACYBP, calcyclin binding protein, GIG5, hCacyBP, PNAS 107, RP1 102G20.6, S100A6 binding protein, S100A6BP, Siah interacting protein, SIP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACYBP Monoclonal antibody (68161-1-Ig)

    WB analysis using 68161-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of 4T1 cells using CACYBP Monoclonal antibody (68161-1-Ig)

    WB analysis of 4T1 using 68161-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CACYBP Monoclonal antibody (68161-1-Ig)

    IP experiment of HeLa using 68161-1-Ig

    IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CACYBP Monoclonal antibody (68161-1-Ig)

    IF Staining of HEK-293 using 68161-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHCT 116 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68161-1-Ig targets CACYBP in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CACYBP fusion protein Ag2340 相同性解析による交差性が予測される生物種
Full Name calcyclin binding protein
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight26-30 kDa
GenBank accession numberBC022352
Gene symbol CACYBP
Gene ID (NCBI) 27101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

Protocols

Product Specific Protocols
WB protocol for CACYBP antibody 68161-1-IgDownload protocol
IF protocol for CACYBP antibody 68161-1-IgDownload protocol
IP protocol for CACYBP antibody 68161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols