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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACYBP Monoclonal antibody (68161-1-Ig)

    WB analysis using 68161-1-Ig (same clone as 68161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using CACYBP Monoclonal antibody (68161-1-Ig)

    WB analysis of 4T1 using 68161-1-Ig (same clone as 68161-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CACYBP Monoclonal antibody (68161-1-Ig)

    IP experiment of HeLa using 68161-1-Ig (same clone as 68161-1-PBS)

    IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACYBP Monoclonal antibody (68161-1-Ig)

    IHC staining of mouse brain using 68161-1-Ig (same clone as 68161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68161-1-Ig (CACYBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACYBP Monoclonal antibody (68161-1-Ig)

    IHC staining of mouse brain using 68161-1-Ig (same clone as 68161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68161-1-Ig (CACYBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CACYBP Monoclonal antibody (68161-1-Ig)

    IF Staining of HEK-293 using 68161-1-Ig (same clone as 68161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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CACYBP Monoclonal antibody, PBS Only

CACYBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68161-1-PBS
Clone No.3C2G11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68161-1-PBS targets CACYBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CACYBP fusion protein Ag2340 相同性解析による交差性が予測される生物種
Full Name calcyclin binding protein
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight26-30 kDa
GenBank accession numberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HB71
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).