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  • Featured Product
  • KD/KO Validated

CAD Polyclonal antibody, PBS Only

CAD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16617-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CAD Polyclonal antibody (16617-1-AP)

    WB analysis of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP (CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CAD Polyclonal antibody (16617-1-AP)

    IP experiment of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    IP result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CAD Polyclonal antibody (16617-1-AP)

    IHC staining of mouse liver using 16617-1-AP (same clone as 16617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CAD Polyclonal antibody (16617-1-AP)

    IHC staining of human liver cancer using 16617-1-AP (same clone as 16617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CAD Polyclonal antibody (16617-1-AP)

    IF Staining of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16617-1-PBS targets CAD in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAD fusion protein Ag9912 相同性解析による交差性が予測される生物種
Full Name carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Calculated molecular weight 2225 aa, 243 kDa
Observed molecular weight 240 kDa
GenBank accession numberBC014178
Gene symbol CAD
Gene ID (NCBI) 790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).