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Validation Data Gallery

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  • Western Blot (WB) analysis of TT cells using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    WB analysis of TT using 68774-1-Ig

    TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CALCA/Calcitonin Monoclonal antibody

CALCA/Calcitonin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68774-1-Ig
Clone No.1A12A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CALCA/CGRP, CALCA, Calcitonin, 1A12A1, CALC1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTT cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68774-1-Ig targets CALCA/Calcitonin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CALCA/Calcitonin fusion protein Ag26141 相同性解析による交差性が予測される生物種
Full Name Calcitonin
Calculated molecular weight 141 aa, 15 kDa
Observed molecular weight14 kDa
GenBank accession numberBC093753
Gene Symbol CALCA
Gene ID (NCBI) 796
RRIDAB_3670424
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.

Protocols

Product Specific Protocols
WB protocol for CALCA/Calcitonin antibody 68774-1-IgDownload protocol
IHC protocol for CALCA/Calcitonin antibody 68774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols