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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CALCA/CGRP Monoclonal antibody

CALCA/CGRP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A1

Cat no : 68774-1-Ig

Synonyms

Alpha type CGRP, CALC1, CALCA, CGRP, CGRP I, CGRP1, CT, KC

Validation Data Gallery

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  • Western Blot (WB) analysis of TT cells using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    WB analysis of TT using 68774-1-Ig

    TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inTT cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68774-1-Ig targets CALCA/CGRP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CALCA/CGRP fusion protein Ag26141 相同性解析による交差性が予測される生物種
Full Name calcitonin-related polypeptide alpha
Calculated molecular weight 141 aa, 15 kDa
Observed molecular weight14 kDa
GenBank accession numberBC093753
Gene symbol CALCA
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).

Protocols

Product Specific Protocols
WB protocol for CALCA/CGRP antibody 68774-1-IgDownload protocol
IHC protocol for CALCA/CGRP antibody 68774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols