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Validation Data Gallery

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  • Western Blot (WB) analysis of TT cells using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    WB analysis of TT using 68774-1-Ig (same clone as 68774-1-PBS)

    TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

  • Sample test of MP50293-1

    Sample test of MP50293-1

    Cytometric bead array in cell lysate using MP50293-1, CALCA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68774-1-PBS. Detection antibody: 68774-2-PBS. Cell lysate: TT, HepG2.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    IHC staining of human thyroid cancer using 68774-1-Ig (same clone as 68774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CALCA/CGRP Monoclonal antibody (68774-1-Ig)

    IHC staining of human thyroid cancer using 68774-1-Ig (same clone as 68774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50293-1

    Cytometric bead array standard curve of MP50293-1

    Cytometric bead array standard curve of MP50293-1, CALCA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68774-1-PBS. Detection antibody: 68774-2-PBS. Standard:Ag26141. Range: 0.781-100 ng/mL.

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CALCA/CGRP Monoclonal antibody, PBS Only (Capture)

CALCA/CGRP Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 68774-1-PBS
Clone No.1A12A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

Calcitonin, CALCA, CALC1, Alpha-type CGRP, Alpha type CGRP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68774-1-PBS targets CALCA/CGRP as part of a matched antibody pair:

MP50293-1: 68774-1-PBS capture and 68774-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CALCA/CGRP fusion protein Ag26141 相同性解析による交差性が予測される生物種
Full Name calcitonin-related polypeptide alpha
Calculated molecular weight 141 aa, 15 kDa
GenBank accession numberBC093753
Gene Symbol CGRP
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06881
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.