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  • Featured Product
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NDP52 Monoclonal antibody

NDP52 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F2C2

Cat no : 66401-1-Ig

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDP52 Monoclonal antibody (66401-1-Ig)

    WB analysis using 66401-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDP52 Monoclonal antibody (66401-1-Ig)

    WB analysis of HeLa using 66401-1-Ig

    WB result of NDP52 antibody (66401-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDP52 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using NDP52 Monoclonal antibody (66401-1-Ig)

    WB analysis of HeLa using 66401-1-Ig

    HeLa, Jurkat cells and human skeletal muscle tissue lysate were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NDP52 Monoclonal antibody (66401-1-Ig)

    IHC staining of human ovary tumor using 66401-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NDP52 Monoclonal antibody (66401-1-Ig)

    IHC staining of human ovary tumor using 66401-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using NDP52 Monoclonal antibody (66401-1-Ig)

    IF Staining of Jurkat using 66401-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NDP52 antibody (66401-1-Ig, Clone: 2F2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, Caco-2 cells, MDA-MB-231 cells, Jurkat cells, MOLT-4 cells, K-562 cells, Karpas-299 cells, human skeletal muscle tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66401-1-Ig targets NDP52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDP52 fusion protein Ag2866 相同性解析による交差性が予測される生物種
Full Name calcium binding and coiled-coil domain 2
Calculated molecular weight 446 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015893
Gene symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_2881775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
WB protocol for NDP52 antibody 66401-1-IgDownload protocol
IHC protocol for NDP52 antibody 66401-1-IgDownload protocol
IF protocol for NDP52 antibody 66401-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Authors - Norihiko Furuya
View Article
humanWB

Autophagy

Copper-dependent autophagic degradation of GPX4 drives ferroptosis

Authors - Qian Xue
View Article
human,mouseWB

mBio

The nonstructural protein 1 of respiratory syncytial virus hijacks host mitophagy as a novel mitophagy receptor to evade the type I IFN response in HEp-2 cells

Authors - Jing Cheng
View Article