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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDP52 Monoclonal antibody (66401-1-Ig)

    WB analysis using 66401-1-Ig (same clone as 66401-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NDP52 Monoclonal antibody (66401-1-Ig)

    WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB result of NDP52 antibody (66401-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDP52 transfected HeLa cells. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NDP52 Monoclonal antibody (66401-1-Ig)

    WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    HeLa, Jurkat cells and human skeletal muscle tissue lysate were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NDP52 Monoclonal antibody (66401-1-Ig)

    IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NDP52 Monoclonal antibody (66401-1-Ig)

    IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using NDP52 Monoclonal antibody (66401-1-Ig)

    IF Staining of Jurkat using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NDP52 antibody (66401-1-Ig, Clone: 2F2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NDP52 Monoclonal antibody, PBS Only

NDP52 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66401-1-PBS
Clone No.2F2C2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66401-1-PBS targets NDP52 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDP52 fusion protein Ag2866 相同性解析による交差性が予測される生物種
Full Name calcium binding and coiled-coil domain 2
Calculated molecular weight 446 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_2881775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13137
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,