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  • Featured Product
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CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E1C11

Cat no : 66693-1-Ig

Synonyms

Caldesmon, CDM, caldesmon 1, CAD, 1E1C11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALD1 Monoclonal antibody (66693-1-Ig)

    WB analysis using 66693-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CALD1 Monoclonal antibody (66693-1-Ig)

    WB analysis of HeLa using 66693-1-Ig

    WB result of CALD1 antibody (66693-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALD1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CALD1 Monoclonal antibody (66693-1-Ig)

    WB analysis using 66693-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human colon cancer using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human colon cancer using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human hysteromyoma using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human hysteromyoma using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of mouse colon using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of mouse colon using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CALD1 Monoclonal antibody (66693-1-Ig)

    IF Staining of HeLa using 66693-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CALD1 Monoclonal antibody (66693-1-Ig)

    IF Staining of HepG2 using 66693-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using CALD1 Monoclonal antibody (66693-1-Ig)

    FC experiment of HeLa using 66693-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CALD1 (66693-1-Ig, Clone:1E1C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inNCI-H1299 cells, HeLa cells, U2OS cells, HepG2 cells, PC-3 cells, SKOV-3 cells, A2780 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, Calu-1 cells, Caco-2 cells, Caki-1 cells, MCF-10A cells, LNCaP cells, SK-N-SH cells
Positive IHC detected inhuman colon cancer tissue, human hysteromyoma tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66693-1-Ig targets CALD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CALD1 fusion protein Ag13676 相同性解析による交差性が予測される生物種
Full Name caldesmon 1
Calculated molecular weight 538 aa, 63 kDa
Observed molecular weight 70 kDa~75 kDa
GenBank accession numberBC040354
Gene symbol CALD1
Gene ID (NCBI) 800
RRIDAB_2882047
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CALD1 antibody 66693-1-IgDownload protocol
IHC protocol for CALD1 antibody 66693-1-IgDownload protocol
IF protocol for CALD1 antibody 66693-1-IgDownload protocol
FC protocol for CALD1 antibody 66693-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Changes in the Expression of Smooth Muscle Cell-Related Genes in Human Dermal Sheath Cup Cells Associated with the Treatment Outcome of Autologous Cell-Based Therapy for Male and Female Pattern Hair Loss.

Authors - Yuzo Yoshida
View Article
humanWB

Int J Clin Pract

GPX3-Mediated Oxidative Stress Affects Pyrimidine Metabolism Levels in Stomach Adenocarcinoma via the AMPK/mTOR Pathway

Authors - Yaowen Zhang
View Article