• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CALD1 Monoclonal antibody, PBS Only

CALD1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1C11

Cat no : 66693-1-PBS

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using CALD1 Monoclonal antibody (66693-1-Ig)

    WB analysis of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

    WB result of CALD1 antibody (66693-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALD1 transfected HeLa cells. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CALD1 Monoclonal antibody (66693-1-Ig)

    WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CALD1 Monoclonal antibody (66693-1-Ig)

    IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CALD1 Monoclonal antibody (66693-1-Ig)

    IF Staining of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CALD1 Monoclonal antibody (66693-1-Ig)

    IF Staining of HepG2 using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66693-1-PBS targets CALD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CALD1 fusion protein Ag13676 相同性解析による交差性が予測される生物種
Full Name caldesmon 1
Calculated molecular weight 538 aa, 63 kDa
Observed molecular weight 70 kDa~75 kDa
GenBank accession numberBC040354
Gene symbol CALD1
Gene ID (NCBI) 800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.