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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CAMK2 Monoclonal antibody

CAMK2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3B3

Cat no : CL488-66843

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, KIAA0968

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated CAMK2 Monoclonal ant (CL488-66843)

    WB analysis using CL488-66843

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66843 (CAMK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66843 targets CAMK2 in WB applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAMK2 fusion protein Ag4736 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase II alpha
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC040457
Gene symbol CaMKII alpha
Gene ID (NCBI) 815
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 CAMK2 antibody CL488-66843Download protocol
Standard Protocols
Click here to view our Standard Protocols