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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAP1 Monoclonal antibody

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H3A10

Cat no : 68207-1-Ig

Synonyms

CAP, CAP 1, CAP1, CAP1 PEN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis using 68207-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of pig lung tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis of pig lung using 68207-1-Ig

    pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    WB analysis of human placenta using 68207-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    IHC staining of human colon cancer using 68207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CAP1 Monoclonal antibody (68207-1-Ig)

    IHC staining of mouse testis using 68207-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAP1 Monoclonal antibody (68207-1-Ig)

    IF Staining of HepG2 using 68207-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAP1 Monoclonal antibody (68207-1-Ig)

    IF Staining of HepG2 using 68207-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAP1 Monoclonal antibody (68207-1-Ig)

    IF Staining of HeLa using 68207-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue, pig lung tissue, human placenta tissue, mouse lung tissue, rat lung tissue, rabbit lung tissue
Positive IHC detected inhuman colon cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68207-1-Ig targets CAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAP1 fusion protein Ag8876 相同性解析による交差性が予測される生物種
Full Name CAP, adenylate cyclase-associated protein 1 (yeast)
Calculated molecular weight 475 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC013963
Gene symbol CAP1
Gene ID (NCBI) 10487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.

Protocols

Product Specific Protocols
WB protocol for CAP1 antibody 68207-1-IgDownload protocol
IHC protocol for CAP1 antibody 68207-1-IgDownload protocol
IF protocol for CAP1 antibody 68207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Int Immunopharmacol

Single-cell RNA sequencing reveals cell-cell communication and potential biomarker in sepsis and septic shock patients

Authors - Guilin Li
View Article
humanIF

Acta Neuropathol

Enhanced microglial dynamics and a paucity of tau seeding in the amyloid plaque microenvironment contribute to cognitive resilience in Alzheimer's disease

Authors - Nur Jury-Garfe
View Article