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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CAP1 Monoclonal anti (CL488-68207)

    IF Staining of HepG2 using CL488-68207

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CAP1 Monoclonal anti (CL488-68207)

    IF Staining of HepG2 using CL488-68207

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated CAP1 Monoclonal antibody

CAP1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68207
Clone No.2H3A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC

Synonyms

CAP, CAP 1, CAP1, CAP1 PEN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68207 targets CAP1 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAP1 fusion protein Ag8876 相同性解析による交差性が予測される生物種
Full Name CAP, adenylate cyclase-associated protein 1 (yeast)
Calculated molecular weight 475 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC013963
Gene Symbol CAP1
Gene ID (NCBI) 10487
RRIDAB_3084441
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01518
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CAP1 antibody CL488-68207Download protocol
FC protocol for CL Plus 488 CAP1 antibody CL488-68207Download protocol
Standard Protocols
Click here to view our Standard Protocols