Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66977 targets CAPN2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CAPN2 fusion protein Ag28427 相同性解析による交差性が予測される生物種 |
| Full Name | calpain 2, (m/II) large subunit |
| Calculated molecular weight | 700 aa, 80 kDa |
| Observed molecular weight | 72-80 kDa |
| GenBank accession number | BC021303 |
| Gene Symbol | CAPN2 |
| Gene ID (NCBI) | 824 |
| RRID | AB_3084740 |
| Conjugate | CoraLite® Plus 594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 594 nm / 615 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P17655 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 594 CAPN2 antibody CL594-66977 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |