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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6B10F11

Cat no : CL594-66352

Synonyms

GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1, caprin 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CAPRIN1 Monoclonal antibod (CL594-66352)

    IF Staining of HeLa using CL594-66352

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated CAPRIN1 antibody (CL594-66352, Clone: 6B10F11 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CAPRIN1 Monoclonal antibod (CL594-66352)

    IF Staining of HeLa using CL594-66352

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CAPRIN1 antibody (CL594-66352, Clone: 6B10F11 ) at dilution of 1:800.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CAPRIN1 Monoclonal antibod (CL594-66352)

    IF Staining of HeLa using CL594-66352

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66352 (CAPRIN1 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®594-conjugated CAPRIN1 Monoclonal antibod (CL594-66352)

    FC experiment of NIH/3T3 using CL594-66352

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CAPRIN1 (CL594-66352, Clone:6B10F11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66352 targets CAPRIN1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7413 相同性解析による交差性が予測される生物種
Full Name cell cycle associated protein 1
Calculated molecular weight 78 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC001731
Gene symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2883566
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Protocols

Product Specific Protocols
IF protocol for CL594 CAPRIN1 antibody CL594-66352Download protocol
Standard Protocols
Click here to view our Standard Protocols