CoraLite® Plus 488-conjugated CARD11 Monoclonal antibody
CARD11 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3F5H6
Cat no : CL488-68269
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68269 targets CARD11 in IF applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CARD11 fusion protein Ag16468 相同性解析による交差性が予測される生物種 |
| Full Name | caspase recruitment domain family, member 11 |
| Calculated molecular weight | 1154 aa, 133 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC111719 |
| Gene symbol | CARD11 |
| Gene ID (NCBI) | 84433 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CARD11 antibody CL488-68269 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
