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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARD9 Monoclonal antibody

CARD9 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F3E10

Cat no : 68657-1-Ig

Synonyms

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis using 68657-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis of U-937 using 68657-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TF-1 cells using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis of TF-1 using 68657-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis using 68657-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CARD9 Monoclonal antibody (68657-1-Ig)

    IF Staining of HeLa using 68657-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

Positive WB detected inNK-92 cells, HL-60 cells, TF-1 cells, U-937 cells, THP-1 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68657-1-Ig targets CARD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CARD9 fusion protein Ag33643 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 9
Calculated molecular weight 62 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC008877
Gene symbol CARD9
Gene ID (NCBI) 64170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

Protocols

Product Specific Protocols
WB protocol for CARD9 antibody 68657-1-IgDownload protocol
IF protocol for CARD9 antibody 68657-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols