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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis of THP-1 using 68657-1-Ig (same clone as 68657-1-PBS)

    WB result of CARD9 antibody (68657-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis of U-937 using 68657-1-Ig (same clone as 68657-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of TF-1 cells using CARD9 Monoclonal antibody (68657-1-Ig)

    WB analysis of TF-1 using 68657-1-Ig (same clone as 68657-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CARD9 Monoclonal antibody (68657-1-Ig)

    IF Staining of HeLa using 68657-1-Ig (same clone as 68657-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CARD9 Monoclonal antibody, PBS Only

CARD9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68657-1-PBS
Clone No.2F3E10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68657-1-PBS targets CARD9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CARD9 fusion protein Ag33643 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 9
Calculated molecular weight 62 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC008877
Gene Symbol CARD9
Gene ID (NCBI) 64170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H257
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.