Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10380-1-AP
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10380-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10380-1-AP
Staurosporine treat Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10380-1-AP
Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10380-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10380-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10380-1-AP
IP result of anti-Caspase 9/p35/p10 (IP:10380-1-AP, 3ug; Detection:10380-1-AP 1:200) with HeLa cells lysate 2500 ug.
IP experiment of HeLa using 10380-1-AP
IP result of anti-Caspase 9/p35/p10 (IP:10380-1-AP, 4ug; Detection:10380-1-AP 1:500) with HeLa cells lysate 4000ug.
IHC staining of human heart using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse lung using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 (under 10x lens).
IHC staining of mouse lung using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 (under 40x lens).
IF Staining of HepG2 using 10380-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 9/p35/p10 antibody (10380-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10380-1-AP

Publications(802)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (8)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

CASP9, Caspase 9, Apoptotic protease-activating factor 3, Apoptotic protease Mch-6, Apoptotic protease Mch 6

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (802)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, HL-60 cells, Staurosporine treated Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse lung tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:300-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 767 publications below
IHC	See 36 publications below
IF	See 15 publications below
ELISA	See 1 publications below
RIP	See 1 publications below

Product Information

10380-1-AP targets Caspase 9/p35/p10 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, zebrafish, hamster, sheep, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 9/p35/p10 fusion protein Ag0404 相同性解析による交差性が予測される生物種
Full Name	caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight	46 kDa
Observed molecular weight	46 kDa, 30-39 kDa
GenBank accession number	BC002452
Gene Symbol	Caspase 9
Gene ID (NCBI)	842
RRID	AB_2068632
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55211
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Protocols

Product Specific Protocols
WB protocol for Caspase 9/p35/p10 antibody 10380-1-AP	Download protocol
IHC protocol for Caspase 9/p35/p10 antibody 10380-1-AP	Download protocol
IF protocol for Caspase 9/p35/p10 antibody 10380-1-AP	Download protocol
IP protocol for Caspase 9/p35/p10 antibody 10380-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma Authors - Lei Xu View Article
mouse	WB,IHC	Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection Authors - Yumei Que View Article
rat	WB	Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model Authors - Mengyan Hu View Article
human	WB	Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. Authors - Xiaowei Zhang View Article
human	WB	Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer Authors - Shaofeng Duan View Article
rat	WB	Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. Authors - Zhi Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10380-1-AP

WB analysis of HL-60 using 10380-1-AP

WB analysis of Jurkat using 10380-1-AP

WB analysis of Jurkat using 10380-1-AP

WB analysis of Jurkat using 10380-1-AP

WB analysis of HeLa using 10380-1-AP

IP experiment of HeLa using 10380-1-AP

IP experiment of HeLa using 10380-1-AP

IHC staining of human heart using 10380-1-AP

IHC staining of human heart using 10380-1-AP

IHC staining of mouse lung using 10380-1-AP

IHC staining of mouse lung using 10380-1-AP

IHC staining of mouse lung using 10380-1-AP

IHC staining of mouse lung using 10380-1-AP

IF Staining of HepG2 using 10380-1-AP

Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.