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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10380

Synonyms

Caspase 9, Caspase 9/p35/p10, CASP9, CASP-9, APAF 3, APAF3, APAF-3, Apoptotic protease Mch 6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP 9, CASPASE 9c, Caspase9, Caspase-9, Caspase-9 subunit p10, Caspase-9 subunit p35, EC:3.4.22.62, ICE LAP6, ICE like apoptotic protease 6, ICELAP6, ICE-LAP6, ICE-like apoptotic protease 6, MCH 6, MCH6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated Caspase 9/p35/p10 Po (CL647-10380)

    FC experiment of HepG2 using CL647-10380

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Caspase 9/p35/p10 (CL647-10380) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10380 targets Caspase 9/p35/p10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 9/p35/p10 fusion protein Ag0404 相同性解析による交差性が予測される生物種
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 35 kDa
GenBank accession numberBC002452
Gene symbol Caspase 9
Gene ID (NCBI) 842
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Caspase 9/p35/p10 antibody CL647-10380Download protocol
Standard Protocols
Click here to view our Standard Protocols