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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBLB Monoclonal antibody

CBLB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3A2

Cat no : 66353-1-Ig

Synonyms

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using CBLB Monoclonal antibody (66353-1-Ig)

    WB analysis of RAW 264.7 using 66353-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using CBLB Monoclonal antibody (66353-1-Ig)

    WB analysis of rat heart using 66353-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using CBLB Monoclonal antibody (66353-1-Ig)

    IHC staining of human heart using 66353-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CBLB Monoclonal antibody (66353-1-Ig)

    IHC staining of human heart using 66353-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CBLB Monoclonal antibody (66353-1-Ig)

    IHC staining of human testis using 66353-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CBLB Monoclonal antibody (66353-1-Ig)

    IHC staining of human testis using 66353-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CBLB Monoclonal antibody (66353-1-Ig)

    IF Staining of RAW 264.7 using 66353-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inRAW 264.7 cells, rat heart tissue
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66353-1-Ig targets CBLB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen CBLB fusion protein Ag18757 相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Calculated molecular weight 982 aa, 109 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC032851
Gene symbol CBLB
Gene ID (NCBI) 868
RRIDAB_2881733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CBLB antibody 66353-1-IgDownload protocol
IHC protocol for CBLB antibody 66353-1-IgDownload protocol
IF protocol for CBLB antibody 66353-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

J Exp Clin Cancer Res

GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway

Authors - Haijun Bao
View Article