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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CoraLite®594-conjugated CBLB Monoclonal antibody (CL594-66353)

    IF Staining of RAW 264.7 using CL594-66353

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite®594 CBLB antibody (CL594-66353, Clone: 2D3A2 ) at dilution of 1:100. DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite®594-conjugated CBLB Monoclonal antibody (CL594-66353)

    IF Staining of U2OS using CL594-66353

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 CBLB antibody (CL594-66353, Clone: 2D3A2 ) at dilution of 1:200.

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CoraLite®594-conjugated CBLB Monoclonal antibody

CBLB Monoclonal Antibody for IF/ICC
Cat No. CL594-66353
Clone No.2D3A2

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

IF/ICC

Synonyms

CBLB, DKFZp686J10223, DKFZp779A0729, DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inRAW 264.7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66353 targets CBLB in IF/ICC applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen CBLB fusion protein Ag18757 相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Calculated molecular weight 982 aa, 109 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC032851
Gene Symbol CBLB
Gene ID (NCBI) 868
RRIDAB_2919978
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13191
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 CBLB antibody CL594-66353Download protocol
Standard Protocols
Click here to view our Standard Protocols