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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBLL1 Polyclonal antibody, PBS Only

CBLL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29219-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBLL1 Polyclonal antibody (29219-1-AP)

    WB analysis using 29219-1-AP (same clone as 29219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29219-1-AP (CBLL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29219-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CBLL1 Polyclonal antibody (29219-1-AP)

    IHC staining of human lung cancer using 29219-1-AP (same clone as 29219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29219-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29219-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29219-1-PBS targets CBLL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBLL1 fusion protein Ag30900 相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC027460
Gene symbol CBLL1
Gene ID (NCBI) 79872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)