Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| RIP | See 1 publications below |
Product Information
11950-1-AP targets CBP20 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CBP20 fusion protein Ag2550 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear cap binding protein subunit 2, 20kDa |
| Calculated molecular weight | 156 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC001255 |
| Gene Symbol | NCBP2 |
| Gene ID (NCBI) | 22916 |
| RRID | AB_2150142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52298 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBP20 antibody 11950-1-AP | Download protocol |
| IHC protocol for CBP20 antibody 11950-1-AP | Download protocol |
| IF protocol for CBP20 antibody 11950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. | ||
Eur J Med Res N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma | ||
Heliyon CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B | ||