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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CBP20 Polyclonal antibody

CBP20 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11950

Synonyms

NCBP2, NCBP-interacting protein 1, NCBP 20 kDa subunit, Cell proliferation-inducing gene 55 protein, CBC2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CBP20 Polyclonal ant (CL488-11950)

    IF Staining of HeLa using CL488-11950

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CBP20 antibody (CL488-11950) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CBP20 Polyclonal ant (CL488-11950)

    FC experiment of HeLa using CL488-11950

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CBP20 Polyclonal antibody (CL488-11950)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11950 targets CBP20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBP20 fusion protein Ag2550 相同性解析による交差性が予測される生物種
Full Name nuclear cap binding protein subunit 2, 20kDa
Calculated molecular weight 156 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC001255
Gene symbol NCBP2
Gene ID (NCBI) 22916
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CBP20 antibody CL488-11950Download protocol
FC protocol for CL Plus 488 CBP20 antibody CL488-11950Download protocol
Standard Protocols
Click here to view our Standard Protocols