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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    WB analysis using 83212-4-RR (same clone as 83212-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    IHC staining of human ovary cancer using 83212-4-RR (same clone as 83212-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83212-4-RR (NCBP1/CBP80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    IF Staining of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    FC experiment of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83212-4-RR

    Affinity and Kinetic Characterization of 83212-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

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NCBP1/CBP80 Recombinant antibody, PBS Only

NCBP1/CBP80 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83212-4-PBS
Clone No.240012G8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

NCBP1, NCBP 80 kDa subunit, NCBP, CBP80, 80 kDa nuclear cap-binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83212-4-PBS targets NCBP1/CBP80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NCBP1/CBP80 fusion protein Ag0496 相同性解析による交差性が予測される生物種
Full Name nuclear cap binding protein subunit 1, 80kDa
Calculated molecular weight 92 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC001450
Gene Symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09161
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.