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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBP80 Recombinant antibody, PBS Only

CBP80 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240012G8

Cat no : 83212-4-PBS

Synonyms

CBP80, NCBP, NCBP 80 kDa subunit, NCBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBP80 Recombinant antibody (83212-4-RR)

    WB analysis using 83212-4-RR (same clone as 83212-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBP80 Recombinant antibody (83212-4-RR)

    IF Staining of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CBP80 Recombinant antibody (83212-4-RR)

    FC experiment of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83212-4-PBS

    Affinity and Kinetic Characterization of 83212-4-PBS

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83212-4-PBS targets CBP80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBP80 fusion protein Ag0496 相同性解析による交差性が予測される生物種
Full Name nuclear cap binding protein subunit 1, 80kDa
Calculated molecular weight 92 kDa
GenBank accession numberBC001450
Gene symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.