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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCBP1/CBP80 Recombinant antibody

NCBP1/CBP80 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240012G8

Cat no : 83212-4-RR

Synonyms

NCBP1, NCBP 80 kDa subunit, NCBP, CBP80, 80 kDa nuclear cap-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBP80 Recombinant antibody (83212-4-RR)

    WB analysis using 83212-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    IHC staining of human ovary cancer using 83212-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83212-4-RR (NCBP1/CBP80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    IF Staining of HepG2 using 83212-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using NCBP1/CBP80 Recombinant antibody (83212-4-RR)

    FC experiment of HepG2 using 83212-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83212-4-RR

    Affinity and Kinetic Characterization of 83212-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, A431 cells, NCI-H1299 cells, PC-12 cells, HEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83212-4-RR targets NCBP1/CBP80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NCBP1/CBP80 fusion protein Ag0496 相同性解析による交差性が予測される生物種
Full Name nuclear cap binding protein subunit 1, 80kDa
Calculated molecular weight 92 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC001450
Gene symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Protocols

Product Specific Protocols
WB protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
IHC protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
IF protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
FC protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols