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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBX1 Recombinant antibody (83248-3-RR)

    WB analysis using 83248-3-RR (same clone as 83248-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-3-RR (CBX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CBX1 Recombinant antibody (83248-3-RR)

    FC experiment of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CBX1 Recombinant antibody (83248-3-RR, Clone:240075E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83248-3-RR

    Affinity and Kinetic Characterization of 83248-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-3-RR against Human CBX1 were performed. The affinity constant is 3.31 nM.

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CBX1 Recombinant antibody, PBS Only

CBX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83248-3-PBS
Clone No.240075E1

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83248-3-PBS targets CBX1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBX1 fusion protein Ag34901 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated molecular weight 27 kDa
GenBank accession numberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.