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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBX1 Recombinant antibody

CBX1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240075E1

Cat no : 83248-3-RR

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBX1 Recombinant antibody (83248-3-RR)

    WB analysis using 83248-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-3-RR (CBX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of HeLa using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of HeLa using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of A431 using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CBX1 Recombinant antibody (83248-3-RR)

    IF Staining of A431 using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CBX1 Recombinant antibody (83248-3-RR)

    FC experiment of HeLa using 83248-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CBX1 Recombinant antibody (83248-3-RR, Clone:240075E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83248-3-RR

    Affinity and Kinetic Characterization of 83248-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-3-RR against Human CBX1 were performed. The affinity constant is 3.31 nM.

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Tested Applications

Positive WB detected inCHO cells, PC-12 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells, A431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83248-3-RR is CBX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBX1 fusion protein Ag34901 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated molecular weight 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC002609
Gene symbol CBX1
Gene ID (NCBI) 10951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

Protocols

Product Specific Protocols
WB protocol for CBX1 antibody 83248-3-RRDownload protocol
IF protocol for CBX1 antibody 83248-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols